High titer, high quality stable cell lines
Our experienced team uses the CHO Edge System and state-of-the-art workflows to perform CLD and upstream process development.
Higher titer cell lines
- Typical titers of 7-11 g/L or higher across modalities, enabled by our data-driven approach to vector design, proprietary transposase, and clone screening methods.
5 g/L or it's free
- We guarantee high titer cell lines for monoclonal IgG antibodies. If the RCB generated for a mAb is under 5 g/L, the CLD campaign and all commercial-use rights are free.
A wealth of experience
- Our team comprises scientists with combined decades of experience developing clinical-grade cell lines for a wide range of molecules, and helping bring these products to market.
How it works
Sequence transfer
You request a cell line development campaign for a specific biologic and provide us with an amino acid sequence. We use Kernel, our proprietary software, for codon optimization, vector design, and biophysical simulation to make a panel of expression vectors.
CLD Start
Stable pool generation
Optimized vectors are used to make stable pools which are assessed for titer and product quality attributes.
In-house analytical development team characterizes key aspects of the product (e.g. charge variance).
1 month
Single cell printing and clone screening
The lead pool is cloned out with monoclonality verification via pre- and post-deposition imaging using the Cytena F-sightTM and Solentim Cell Metric®.
2 months
Clones characterized and banked
Our proprietary screening method is used to enrich for high-performance clones.
Critical quality attributes are characterized as required by in-house analytical development team.
3 months
Scale-up and Ambr® 15 run
24-48 clones are progressed for fed-batch analysis at Ambr® 15 scale.
Additional process development using Ambr® 250 and bench top bioreactors available. We also offer stability studies and additional analytical characterization upon request.
4 months
Research cell bank release
We ship research cell banks for the top clones to your facility, your preferred CDMO, or our recommended master cell banking and manufacturing partners.
System performance
What you get
Top 5 clones
- Producing your protein therapeutic of interest
>80% titer stability
- Clones maintain stability across 75 generations
CMC-compliant documentation
- Plus a platform process to tech-transfer into your facility
