CHO Edge System

Integrating genetic tools and data-driven models to routinely achieve titers of 7–11 g/L across modalities

A new approach to biologicS production

Reduce the risk of a low titer cell line

Applications

Expression can vary significantly between different molecules using the same vector. Asimov’s CHO Edge System incorporates a GS knock-out CHO host, a hyperactive transposase, a library of >1000 characterized genetic elements and advanced computational tools, enabling exploration of vector design space.

CHO cycle overview graphic

System performance

Exploring vector design space

Selection stringency, heavy-chain/light-chain ratio, signal peptide pairings, and codon optimization all uniquely influence the expression of different molecules. Figure: Stable pool titers for four different molecules from six vector designs; the optimal vector design is unique to the molecule.

Exploring vector design space relative titer chart

High titers across modalities

Routinely achieve 7-11 g/L clones across biologic modalities using the CHO Edge System. Figure: Top 12 clones for six different molecules analyzed in a 14 day Ambr® 15 platform process.

Highly stable clones

Transposase-mediated integration drives >80% titer stability over 75 generations. Figure: Average relative titer of 5-7 clones per molecule across 4 different molecules.

Partnering with Asimov

How to work with us

We provide tools for you to develop a cell line

We will ship the software, cells, and genes you need to design your cell line in-house.

We develop a cell line for you

Using our platform, we will design a custom cell line specifically for your needs and ship it to you.

Cell line delivery

We provide tools for you to develop a cell line

We will ship the software, cells, and genes you need to design your cell line in-house.

Plasmid design interface

You design DNA

You use our software to design and optimize genetic constructs using best-in-class parts and models.

Ship DNA icon

We build DNA

We will ship the software, cells, and genes you need to design your cell line in-house.

DNA transfection into cells

You transfect cells

You transfect and generate stable cell lines and use our software for data analysis.

Cell line selection

You request producer cells

You request a producer cell line for a biologic or gene therapy.

DNA design and development interface

We do cell line development

We do cell line development and process development at our site.

Communication bubbles

We share progress

We share progress and data with you using 
our software.

Cell line delivery

We ship you producer cell banks

We ship you clonal cell banks and transfer the process to your site.

We provide tools for you to develop a cell line

We will ship the software, cells, and genes you need to design your cell line in-house.

We develop a cell line for you

Using our platform, we will design a custom cell line specifically for your needs and ship it to you.

Ship CHO cells icon

We provide tools for you to develop a cell line

We will ship the software, cells, and genes you need to design your cell line in-house.

DNA design icon

You design DNA

You use our software to design and optimize genetic constructs using best-in-class parts and models.

Ship DNA icon

We build DNA

We will ship the software, cells, and genes you need to design your cell line in-house.

CLD performance icon

You transfect cells

You transfect and generate stable cell lines and use our software for data analysis.

CHO cell line request icon

You request producer cells

You request a producer cell line for a biologic or gene therapy.

CLD process development icon

We do cell line development

We do cell line development and process development at our site.

CLD process development icon

We share progress

We share progress and data with you using 
our software.

Up to date icon

We ship you producer cell banks

We ship you clonal cell banks and transfer theprocess to your site.

We develop a cell line for you

Using our platform, we will design a custom cell line specifically for your needs and ship it to you.

Ship CHO cells icon

We provide tools for you to develop a cell line

We will ship the software, cells, and genes you need to design your cell line in-house.

DNA design icon

You design DNA

You use our software to design and optimize genetic constructs using best-in-class parts and models.

Ship DNA icon

We build DNA

We will ship the software, cells, and genes you need to design your cell line in-house.

CLD performance icon

You transfect cells

You transfect and generate stable cell lines and use our software for data analysis.

CHO cell line request icon

You request producer cells

You request a producer cell line for a biologic or gene therapy.

CLD process development icon

We do cell line development

We do cell line development and process development at our site.

Up to date icon

We share progress

We share progress and data with you using 
our software.

Ship stable cell lines icon

We ship you producer cell banks

We ship you clonal cell banks and transfer the process to your site.

Two ways to work with us

We transfer the CHO Edge System to your lab

Ship CHO cells icon

We ship you CHO cells and grant software access

Vials of GMP-banked CHO cells with regulatory documentation are shipped to you, and your team is onboarded into our vector design software, Kernel.

Plasmid design interface

You design DNA

Using our parts and computational models, you design a set of expression vectors. We're here to help if needed.

Ship DNA icon

We ship your DNA

We construct, prepare, sequence, QC, and ship plasmid DNA direct to your facility.

DNA transfection into cells

You perform cell line development

You transfect and generate stable cell lines, with technical support from our team.

We perform CLD services in our lab

Cell line selection

You request a stable CHO cell line

Your cell line development campaign begins with you providing us with an amino acid sequence.

DNA design and development interface

We perform cell line and upstream
process development with CHO Edge

CLD and PD is all done at our site using state-of-the-art equipment and industry-leading timelines.

Communication bubbles

We keep you up to date at every stage

We’ll collaborate and share the latest data to ensure project success.

Cell line delivery

We ship you clonal, stable cell lines

Vials of research cell banks are shipped to your lab, the process is transferred to your site, and documentation for your regulatory filing supplied.

Cell line delivery

We provide tools for you to develop a cell line

We will ship the software, cells, and genes you need to design your cell line in-house.

Plasmid design interface

You design DNA

You use our software to design and optimize genetic constructs using best-in-class parts and models.

Ship DNA icon

We build DNA

We will ship the software, cells, and genes you need to design your cell line in-house.

DNA transfection into cells

You transfect cells

You transfect and generate stable cell lines and use our software for data analysis.

Cell line selection

You request producer cells

You request a producer cell line for a biologic or gene therapy.

DNA design and development interface

We do cell line development

We do cell line development and process development at our site.

Communication bubbles

We share progress

We share progress and data with you using 
our software.

Cell line delivery

We ship you producer cell banks

We ship you clonal cell banks and transfer the process to your site.

We transfer the CHO Edge System to your lab

We develop a cell line for you

Using our platform, we will design a custom cell line specifically for your needs and ship it to you.

Ship CHO cells icon

We ship you host cells and grant software access

Vials of GMP-banked host cells with regulatory documentation are shipped to you, and your team onboarded into our CAD software.

DNA design icon

You design DNA

Using our parts and computational models, you design and optimize expression vectors.

Ship DNA icon

We ship your DNA

We construct, prepare, sequence, QC, and ship plasmid DNA direct to your facility.

CLD performance icon

You perform cell line development

You transfect and generate stable cell lines, with technical support from our team.

CHO cell line request icon

You request producer cells

You request a producer cell line for a biologic or gene therapy.

CLD process development icon

We do cell line development

We do cell line development and process development at our site.

CLD process development icon

We share progress

We share progress and data with you using 
our software.

Up to date icon

We ship you producer cell banks

We ship you clonal cell banks and transfer theprocess to your site.

We perform cell line development services in our lab

Ship CHO cells icon

We provide tools for you to develop a cell line

We will ship the software, cells, and genes you need to design your cell line in-house.

DNA design icon

You design DNA

You use our software to design and optimize genetic constructs using best-in-class parts and models.

Ship DNA icon

We build DNA

We will ship the software, cells, and genes you need to design your cell line in-house.

CLD performance icon

You transfect cells

You transfect and generate stable cell lines and use our software for data analysis.

CHO cell line request icon

You request a stable cell line

Your cell line development campaign begins with you providing us with an amino acid sequence.

CLD process development icon

We perform cell line and upstream process development

CLD and PD is all done at our site using state-of-the-art equipment and industry-leading timelines.

Up to date icon

We collaborate and keep you up to date

At every stage, we’ll collaborate and share the latest data, to ensure project success.

Ship stable cell lines icon

We ship you clonal, stable cell lines

Vials of clonal cell banks are shipped to your lab, and the process is transferred to your site, as well as documentation for your regulatory filing.

Contact us

Learn more about the CHO Edge System

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CONTACT US

We're hiring at the intersection of biology, engineering, and machine learning.

Asimov team at their Boston office
Illustration of a white blood cell